Senators voted 51-49 to reject a motion to call witnesses after a weeks-long battle between Republicans and Democrats.
The opposing sides debated whether a president can legitimately request investigations into a political rival and whether a quid pro quo amounts to an impeachable offense.
Lawmakers pressed for specifics on the president's effort to push Ukraine to announce investigations into former Vice President Joe Biden and his son Hunter.
President Donald Trump's impeachment defense team on Tuesday concluded their third day of oral arguments by trying to undermine leaked details from an upcoming book by former national security adviser John Bolton.
Today's defense unfolded in the aftermath of a leak from an unpublished manuscript by former national security adviser John Bolton.
The Senate approved ground rules for President Donald Trump's impeachment trial that include unexpected concessions addressing some of the Democrats' concerns.